Genetics

field of genetics that attempts to understand the content, organization, function, and evolution of genetic information contained in whole genomes.

Studies the organization and sequence of genetic information contained within a gene

based on recombination frequencies

based on the direct DNA sequence (Map-based sequencing, Whole-genome shotgun sequencing)

emerging field consisting of molecular biology and computer science that centers on developing databases, computer-search algorithms, gene prediction software, and other analytical tool s

First create detailed genetic and physical maps of the genome, which provide known locations of genetic markers at regularly spaced intervals along each chromosome. (laboring extensive)

maps are not needed;rely on a computer

Compares similarities and differences in gene content, function, and organization among genomes of different organisms.

Large area with no known genes

The study of gene functions based on the resulting RNAs or proteins they encode, and considers the functions of other components of the genome, such as regulatory elements.

All the RNA molecules transcribed from a genome

All the proteins encoded by the genome

Monitor gene expression in different tissues or under different conditions (nucleic and hybridization)

Study of the proteome, complete set of proteins found in a given cell

Method for precisely determining the molecular mass of a molecule (mass-to-charge m/z ratio)

changes in DNA sequence that modify restriction enzyme recognition sites
-base changes between individ. will introduce/remove RE site
-results in unique patterns in individ.
-can trace within families

differences in copy number (SSLP, mini satellite, microsatellite)

single base change

altering an organism genome (Genetically modified organisms [GMOs])

Therapeutic technique that aims to transfer normal genes into a patient’s cell. (wont be passed along to next generation b/c its not within the germ line)