Chapter 12

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Bioinformatics
the use of computational tools to acquire, analyze, store, and access DNA and protein sequences
Chromosomal Island
a bacterial chromosome region of foreign origin that contains clustered genes for some extra property such as virulence or symbiosis
Codon Bias
the relative proportions of different codons encoding the same amino acid; it varies in different organisms. same as codon usage
Gene Chip/Microarray
small solid-state supports to which genes or portions of genes are affixed and arrayed spatially in a known pattern
Gene Family
genes related in sequence to each other because of common evolutionary origin
Genome
the total complement of genetic information of a cell or a virus
Genomics
the discipline that maps, sequences, analyzes, and compares genomes
Homologs
genes related in sequence to an extent that implies common genetic ancestry; includes both orthologs and paralogs
Horizontal Gene Transfer
the transfer of genetic information between organisms as opposed to transfer from parent to offspring
Integrase
the enzyme that inserts cassettes into an integron
Integron
a genetic element that collects and express genes carried by cassettes
Interactome
the total set of interactions between proteins – or other macromolecules- in an organism
Metabolome
the total complement of small molecules and metabolic intermediates of a cell or organism
Metagenome
the total complement of all the cells present in a particular environment
Metagenomics
the genomic analysis of pooled DNA or RNA from an environmental sample containing organisms that have not been isolated; same as environmental genomics
Microarray
small, solid-state supports to which genes or portions of genes are affixed and arrayed spatially in a known pattern (also known as gene chips)
Open Reading Frame(ORF)
a sequence of DNA or RNA that could be translated to give a polypeptide
Ortholog
a gene in one organism because of descent from a common ancestor
Paralog
a gene whose similarity to one or more other genes in the same organism is the result of gene duplication
Pathogenicity Island
a bacterial chromosome region of foreign origin that contains clustered genes for virulence
Primer
an oligonucleotide to which DNA polymerase attaches the first deoxyribonucleotide during DNA synthesis
Proteome
the total set of proteins encoded by a genome or the total protein complement of an organism
Proteomics
the genome-wide study of the structure, function, and regulation of the proteins of an organism
RNA editing
changing the coding sequence of an RNA molecule by altering, adding, or removing bases
Sequencing
deducing the order of nucleotides in an DNA or RNA molecule by a series of chemical reactions
Shotgun Sequencing
sequencing of DNA from previously cloned small fragments of a genome in random fashion, followed by computational methods to reconstruct the entire genome sequence
Transcriptome
the complement of all RNA produced in an organism under a specific set of conditions
Categories: Microbiology